Plant Transcription Factor Database
Previous version: v3.0
Spinacia oleracea
HSF Family
Species TF ID Description
Sp_003720_make.t1HSF family protein
Sp_003720_make.t2HSF family protein
Sp_052050_ywuc.t1HSF family protein
Sp_058360_fokh.t1HSF family protein
Sp_083250_khdt.t1HSF family protein
Sp_127680_dkhu.t1HSF family protein
Sp_130020_rokd.t1HSF family protein
Sp_158990_xngr.t1HSF family protein
Sp_176410_amnr.t1HSF family protein
Sp_177340_cfmo.t1HSF family protein
Sp_178240_uwko.t1HSF family protein
Sp_180830_utra.t1HSF family protein
Sp_201140_jppd.t1HSF family protein
Sp_211390_waxk.t1HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645